Nitric oxide affect early embryo development through S-nitrosylation of cytoplasmic protein
Academic Column 2011.04.06
The 50th Annual Congress and international Symposium of Taiwan Association of Obstetrics and Gynecology. (2011 Annual Meeting)
Nitric oxide affect early embryo development through S-nitrosylation of cytoplasmic protein
Tsung-Hsien Lee 123, LIU CHONG XIAN 3, CAO,HUI-MEI 1, HUANG JUN-JIA 1, Zhong- Yi Chen 1, Maw-Sheng Lee 123
1 Division of Infertility Clinic, Lee Women’s Hospital, 2 Institute of Medicine, Chung Shan Medical University, 3 Department of Obstetrics and Gynecology, China Medical University Hospital
Nitric oxide affect early embryo development through S-nitrosylation of cytoplasmic protein
Tsung-Hsien Lee 123, LIU CHONG XIAN 3, CAO,HUI-MEI 1, HUANG JUN-JIA 1, Zhong- Yi Chen 1, Maw-Sheng Lee 123
1 Division of Infertility Clinic, Lee Women’s Hospital, 2 Institute of Medicine, Chung Shan Medical University, 3 Department of Obstetrics and Gynecology, China Medical University Hospital
Specific Aim：To investigate the mechanisms of nitric oxide (NO) in the development and apoptosis of early-stage embryos.
Methods：Observation of early-stage mouse embryo development in in-vitro culture treated with NO donor or NOS inhibitor.
Results：The supplementation of NO donor disrupted embryo development and induced reduction of mitochondrial membrane potential, as well as elevated level of S-nitrosylation of cytoplasmic proteins. The addition of NOS inhibitor arrested embryo development but did not significantly affect the mitochondrial membrane potential, cytoplasmic protein S-nitrosylastion was lower in NOS inhibitor-treated embryos. Supplementation of anti-oxidants into culture medium was able to restore the quality of embryos.
CONCLUSION：The effect of NO donor on embryo development is closely related to the decline of mitochondrial membrane potential, as well as excess cytoplasmic protein S-nitrosylation and induced apoptosis. Supplementation of anti-oxidants into culture medium can help the embryo escape from the deleterious effect of NO.